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T7816

Monoclonal Anti-β-Tubulin I antibody produced in mouse

clone SAP.4G5, ascites fluid

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02095890
Conjugate:
unconjugated
Clone:
SAP.4G5, monoclonal
Application:
ARR, ELISA (i), ICC, IHC (p), WB
Citations:
234
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SAP.4G5, monoclonal

mol wt

antigen ~55 kDa

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:20,000 using cultured chicken fibroblasts

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Related Categories

General description

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Application

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Biochem/physiol Actions

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Information

常规特殊物品
动物来源生物产品
动物来源生物产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000547520
0000547520
0000504575
0000504575
0000439644

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Richard G James et al.
PloS one, 7(12), e50457-e50457 (2012-12-12)
The Wnt/ß-catenin signaling pathway controls important cellular events during development and often contributes to disease when dysregulated. Using high throughput screening we have identified a new small molecule inhibitor of Wnt/ß-catenin signaling, WIKI4. WIKI4 inhibits expression of ß-catenin target genes
Yosef Buganim et al.
Cancer research, 66(22), 10750-10759 (2006-11-17)
Mutations in p53 are ubiquitous in human tumors. Some p53 mutations not only result in loss of wild-type (WT) activity but also grant additional functions, termed "gain of function." In this study, we explore how the status of p53 affects
M C Roach et al.
Cell motility and the cytoskeleton, 39(4), 273-285 (1998-05-15)
Tubulin, the subunit protein of microtubules, is an alpha/beta heterodimer. In many organisms, both alpha and beta consist of various isotypes. Although the isotypes differ in their tissue distributions, the question of whether the isotypes perform different functions in vivo
Ohad Tarcic et al.
Cell reports, 14(6), 1462-1476 (2016-02-09)
Factors linking inflammation and cancer are of great interest. We now report that the chromatin-targeting E3 ubiquitin ligase RNF20/RNF40, driving histone H2B monoubiquitylation (H2Bub1), modulates inflammation and inflammation-associated cancer in mice and humans. Downregulation of RNF20 and H2Bub1 favors recruitment
Richard G James et al.
The Journal of biological chemistry, 288(48), 34658-34670 (2013-10-12)
Advances in phosphoproteomics have made it possible to monitor changes in protein phosphorylation that occur at different steps in signal transduction and have aided the identification of new pathway components. In the present study, we applied this technology to advance
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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Global Trade Item Number

SKUGTIN
T7816-.2ML04061837378652

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