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T8694

TrkB/Fc Chimera human

Name: TrkB/Fc Chimera human
Brand: SIGMA

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

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About This Item

UNSPSC Code:

51111800

NACRES:

NA.32

MDL number:

MFCD03458837

Form:

lyophilized powder

Assay:

>90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in NSO cells

Mol wt:

120-130 kDa by SDS-PAGE (reducing), calculated mol wt 71 kDa

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Properties

biological source

human

Quality Segment

200

recombinant

expressed in NSO cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

potency

0.1-0.4 μg/mL ED₅₀

mol wt

120-130 kDa by SDS-PAGE (reducing), calculated mol wt 71 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

western blot: suitable

impurities

endotoxin, tested

UniProt accession no.

Q16620

storage temp.

−20°C

Gene Information

human ... NTRK2(4915)

Description

Application

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

Biochem/physiol Actions

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Preparation Note

Reconstitute to 100 μg/mL in phosphate buffered saline.

Analysis Note

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

Other Notes

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c