T8768
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
horseradish peroxidase substrate, chromogenic, ≥98%, powder
Synonym(s):
TMB dihydrochloride
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About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
CAS Number:
Molecular Weight:
313.27 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
Product Name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate, powder, ≥98%
Quality Segment
assay
≥98%
form
powder
solubility
acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy
storage temp.
2-8°C
SMILES string
O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2
InChI key
KVCWTKDFVVSVSJ-UHFFFAOYSA-N
General description
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.
Application
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Noncarcinogenic substitute for benzidine.
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wgk
WGK 2
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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