T8819
TFIIB human
~5 units/μL, recombinant, expressed in E. coli, solution
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About This Item
Form:
solution
Recombinant:
expressed in E. coli
Mol wt:
~32 kDa
recombinant
expressed in E. coli
Quality Segment
form
solution
mol wt
~32 kDa
concentration
~5 units/μL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... GTF2B(2959)
Biochem/physiol Actions
General transcription factor for RNA Polymerase II.
The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation.
Physical form
Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.
Other Notes
One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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