T8819
TFIIB human
~5 units/μL, recombinant, expressed in E. coli, solution
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About This Item
Form:
solution
Recombinant:
expressed in E. coli
Mol wt:
~32 kDa
recombinant
expressed in E. coli
Quality Segment
form
solution
mol wt
~32 kDa
concentration
~5 units/μL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... GTF2B(2959)
Biochem/physiol Actions
The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation.
General transcription factor for RNA Polymerase II.
Physical form
Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.
Other Notes
One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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