T9179
Terrific Broth
EZMix powder microbial growth medium
Synonym(s):
TB media
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Level
description
TB; quick dissolve, dust-free formulation
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
application(s)
agriculture
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
Terrific broth has been used:
- as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
- to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
- Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
Features and Benefits
Terrific Broth EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- A budget-friendly alternative to liquid
- Modified formulation (without glycerol)
- Easy scale-up using larger package sizes
Preparation Note
Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
Legal Information
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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Protocols
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| T9179-1KG | 04061837380754 |
| T9179-2X5L | 04061835575053 |
| T9179-6X500ML | 04061837380785 |