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Merck
CN

T9455

TMB Enhanced One Component HRP Membrane Substrate

Synonym(s):

HRP substrate, TMB, detection reagent, detection substrate

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About This Item

NACRES:
NA.83
UNSPSC Code:
12352204
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form

solution

Quality Segment

storage temp.

2-8°C

General description

TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.

Application

TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year at 2-8 °C.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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