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T9577

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit

Name: Anti-p8/TTD-A (N-terminal) antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~8 kDa

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:200-1:400 using paraformaldehyde-fixed HEK-293T cells, over-expressing human p8/TTD-A, western blot: 1:250-1:500 using lysates of CHO or HEK-293T over-expressing human p8/TTD-A

UniProt accession no.

Q6ZYL4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GTF2H5(404672)
mouse ... Gtf2h5(66467)
rat ... Gtf2h5(502227)

General description

8-kDa trichothiodystrophy group A (p8/TTD-A) protein is the tenth subunit of general transcription factor IIH (TGFIIH). It is present as both, bound to transcription factor IIH (TFIIH) as well as a free fraction that shuffles between the cytoplasm and nucleus. The p8/TTD-A gene is located on the human chromosome at 6q25.3.

p8/TTD-A (N-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

Application

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Anti-p8/TTD-A (N-terminal) specifically recognizes human, rat, and mouse p8/TTD-A.

The 8-kDa trichothiodystrophy group A (p8/TTD-A) protein is required for the stability of the transcription factor IIH (TFIIH) complex. The cells from patients with p8/TTD-A have reduced levels of TFIIH while overexpressing p8/TTD-A restored the cellular level TFIIH complex. Defects in the p8/TTD-A gene are responsible for the third group of the sun-sensitive form of trichothiodystrophy (TTD), a rare autosomal recessive disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

108K4771

108K4770

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Abnormal striatal dopaminergic synapses in National NeuroAIDS Tissue Consortium subjects with HIV encephalitis

Gelman B B, et al.

Journal of Neuroimmune Pharmacology, 1(4), 410-420 (2006)

p8/TTD-A as a repair-specific TFIIH subunit

Coin F, et al.

Molecular Cell, 21(2), 215-226 (2006)

Dynamic interaction of TTDA with TFIIH is stabilized by nucleotide excision repair in living cells

Giglia-Mari G, et al.

PLoS Biology, 4(6), e156-e156 (2006)

Correlation between 6q25. 3 deletion status and survival in pediatric intracranial ependymomas

Monoranu C M, et al.

Cancer Genetics and Cytogenetics, 182(1), 18-26 (2008)

Sublimiting concentration of TFIIH transcription/DNA repair factor causes TTD-A trichothiodystrophy disorder

Vermeulen W, et al.

Nature Genetics, 26(3), 307-313 (2000)

Global Trade Item Number

SKU	GTIN
T9577-25UL	04061837772962
T9577-200UL	04061835688081

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