Merck
CN
All Photos(4)

Documents

T9777

Sigma-Aldrich

Trypsin-chymotrypsin inhibitor from Glycine max (soybean)

lyophilized powder

Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
Bowman-Birk Inhibitor
CAS Number:
37330-34-0
MDL number:
MFCD00132532
NACRES:
NA.77

biological source

Glycine max (soybean)

Quality Level

200

form

lyophilized powder

composition

Protein, 70-90% biuret

storage temp.

2-8°C

Related Categories

General description

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.
Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Application

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.

Unit Definition

One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Analysis Note

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS
Ruppenthal S, et al.
PLoS ONE, 13(1), e0191734-e0191734 (2018)
Epitopes resistance to the simulated gastrointestinal digestion of beta-lactoglobulin submitted to two-step enzymatic modification
Villas-Boas M, et al.
Food Research International, 72, 191-197 (2015)
Alfonso Clemente et al.
Molecular nutrition & food research, 54(3), 396-405 (2009-11-04)
Bowman-Birk inhibitors (BBI) from soybean and related proteins are naturally occurring protease inhibitors with potential health-promoting properties within the gastrointestinal tract. In this work, we have investigated the effects of soybean BBI proteins on HT29 colon adenocarcinoma cells, compared with
Yu Liu et al.
Viruses, 10(10) (2018-10-17)
The Bowman‒Birk inhibitor (BBI), a protease inhibitor derived from soybeans, has been extensively studied in anti-tumor and anti-inflammation research. We recently reported that BBI has an anti-HIV-1 property in primary human macrophages. Because HSV-2 infection plays a role in facilitating
The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent
Kennedy AR
American Journal of Clinical Nutrition, 68(6), 1406S-1412S (1998)
View All Related Papers

Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service