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TOX1

In Vitro Toxicology Assay Kit, MTT based

Synonym(s):

mitochondrial activity assay

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About This Item

NACRES:
NA.84
UNSPSC Code:
12352207
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usage

 kit sufficient for 1,000 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

Related Categories

General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

53.6 °F

flash_point_c

12 °C

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
0000519744
0000519744
SLCR1314
SLCR1315
SLCL3638

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J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
Biomaterials, 33(7), 2345-2350 (2012)
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells
Aslamkhan AG, et al.
Molecular Pharmacology, 63(3), 590-596 (2003)
Srijayaprakash B Uppada et al.
International journal of nanomedicine, 9, 43-53 (2014-01-07)
Oligonucleotides homologous to 3'-telomere overhang (T-oligos) trigger inherent telomere-based DNA damage responses mediated by p53 and/or ATM and induce senescence or apoptosis in various cancerous cells. However, T-oligo has limited stability in vivo due to serum and intracellular nucleases. To
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Global Trade Item Number

SKUGTIN
TOX1-1KT04061838074140

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