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TOX6

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Synonym(s):

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

NACRES:
NA.84
UNSPSC Code:
12352207
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form

liquid

Quality Level

200

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

565 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

room temp

General description

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Application

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Biochem/physiol Actions

Dye binds to cellular protein and is then solubilized in base.

signalword

Danger

wgk

WGK 2

pictograms

CorrosionEnvironment
GHS05,GHS09

hcodes

H314,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8A - Combustible corrosive hazardous materials

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000296292
0000296294
0000296292
0000296294
SLCK4527

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Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Sofia Oliveira-Pinto et al.
Bioorganic chemistry, 100, 103942-103942 (2020-05-26)
A selection of new chromeno[2,3-b]pyridines was prepared from chromenylacrylonitriles and N-substituted piperazines, using a novel and efficient synthetic procedure. The compounds were tested for their anticancer activity using breast cancer cell lines MCF-7, Hs578t and MDA-MB-231 and the non-neoplastic cell
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
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细胞活力和增殖测定

基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。

Global Trade Item Number

SKUGTIN
TOX6-1KT04061837397950

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