TOX7
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
form
liquid
Quality Level
usage
kit sufficient for 500 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
protein quantification: suitable
λmax
490 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
−20°C
Related Categories
General description
Application
- to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
- to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
- to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
- for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.
Biochem/physiol Actions
.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
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705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
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How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
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In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
Can TOX7, In Vitro Toxicology Assay kit be used to quantitate the amount of LDH in my samples?
This assay kit was designed as a semi-quantitative assay for LDH. The change in the OD between the treated samples and control samples is to be compared and is generally reported as a fold increase/decrease from the control. The kit does not contain a LDH standard. Product L3916 can be used as an LDH standard for this assay. The detection range (LDH units/ml) for the kit has not been established.
I'm using the TOX7 In Vitro Toxicology Assay Kit. I do not have a 690 nm filter to use for a reference/background. What can I use?
The reference wavelength needs to be greater than the absorbance spectra of the assay. The greater the difference between the assay wavelength and the reference wavelength, the better. A reference wavelength of 590 nm or greater can be used with the substrate for this assay. If a reference wavelength is not available, the assay will work. The use of the reference wavelength increases the signal to noise ratio to give a better assay range.
The TOX7 dye solution has a precipitate present. Can I still use it?
Product No. L2277 LDH Assay Dye Solution is a saturated solution and some precipitation may appear upon thawing. The product will partially re-solubilize when warmed to 37°C and the resulting solution can be decanted away from any remaining precipitate. Precipitated dye added to reactions, or removed from the solution will not affect the performance or results obtained from this kit.
I am using a protease inhibitor cocktail in the cell culture media. Will this interfere with the TOX7 In Vitro Toxicology assay?
The protease inhibitor cocktails do not interfere with LDH activity. Media from cells cultured with protease inhibitors can be used in this assay.
Will Triton X100 interfere with the TOX7 In Vitro Toxicology Kit assay?
Triton X100 will not interfere with TOX7 assay.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can you use tissue explants in product TOX7, In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based?
Unfortunately, we have not tested product TOX7, In Vitro Toxicology Assay Kit, on tissue samples. If the tissue is minced well and can be lysed completely with the lysis buffer for the cells, it may work to determine cell biomass. The following paper modified the assay for use with explants.Benyo, D.F., et al., "Hypoxia Stimulates Cytokine Production by Villous Explants from the Human Placenta", The Journal of Clinical Endocrinology & Metabolism 82(5), 1582-1588 (1997).Assessment of explant viability was routinely monitored by measuring the release of LDH into medium relative to a 1% Triton X-100 (Sigma)-lysed positive control. The assay employed a modification of the TOX7 viability kit supplied by Sigma-Aldrich and entailed adding a 20-fold volume excess of assay mixture (160 mL) to the medium sample tested (8 mL). The assay mixture consisted of equal volumes of substrate (lactate), enzyme (diaphorase), and dye (nitro blue tetrazolium) solutions, and the reaction was allowed to proceed for 20 min in a microtiter plate before the addition of 0.1 vol 1N HCl. Color change was quantified by reading the absorbance at 490 nm, and the percent cell death calculated.
What is the minimum number of cells to use in the LDH assay, Product TOX7, In Vitro Toxicology Assay Kit?
Using Product TOX7, In Vitro Toxicology Assay Kit, the minimum cell number for best response is 2 x 104.
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