Merck
CN
All Photos(1)

Documents

Safety Information

TP0200

Sigma-Aldrich

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification

sufficient for ~50 manual assays

Sign Into View Organizational & Contract Pricing

Select a Size

Change View
NACRES:
NA.32

description

wavelength 500-750nm

Quality Level

usage

sufficient for ~50 manual assays

protein detection level measuring range

0-1 mg/mL

parameter

18-26 °C temperature
200 μL sample volume

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
1.10307PE0230MOD50
Protein Kit photometric, 0.5-10 g/L (Protein), Spectroquant®

Supelco

1.10307

Protein Kit

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

20-25°C

description

wavelength 500-750nm

description

-

description

-

description

-

protein detection level measuring range

0-1 mg/mL

protein detection level measuring range

-

protein detection level measuring range

-

protein detection level measuring range

-

parameter

18-26 °C temperature

parameter

-

parameter

-

parameter

-

General description

The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.

Application

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
  • in the dialyzates/extracts of gliadins
  • in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoaSalvia hispanica L.)
  • in the stored samples of rat liver microsomal fractions

Principle

Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.

Kit Components Also Available Separately

Product No.
Description
SDS

  • B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLSDS

  • Folin & Ciocalteu’s phenol reagent 5 mL

  • Protein standard 5 mL

required but not provided

Product No.
Description
Pricing

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

Supelco

Supelco

P5369

Protein Standard

Bartosz Fotschki et al.
Molecules (Basel, Switzerland), 23(9) (2018-08-24)
The aim of this in vitro study was to examine the effect of raspberry polyphenolic extract on the immune-metabolic molecular mechanisms activated by obesity-related signals in hepatocytes (HB-8965®). Alterations in endosomal/lysosomal activity (neutral red uptake assay, NR), the expression of
A Smialowska et al.
Journal of dairy science, 100(4), 2553-2563 (2017-02-22)
We isolated goat phosphopeptides via calcium and ethanol precipitation from a caseinate digest and investigated their feasibility as an iron-fortification ingredient in nutritional foods. Goat tryptic-digested phosphopeptides could bind 54.37 ± 0.50 mg of Fe/g of protein compared with goat
Jose Laparra et al.
Oncotarget, 10(7), 760-772 (2019-02-19)
Imbalances in innate immunity and the activity of innate immune cells are implicated in the development of hepatocellular carcinoma (HCC). Plant seeds are good sources of protease inhibitors, which can have a significant influence on human health disorders, especially in
David Kessel
Photochemistry and photobiology, 90(5), 1211-1213 (2014-04-26)
When the initial effect of photodynamic therapy (PDT) involves mitochondrial photodamage, an early effect is loss of the mitochondrial membrane potential (ΔΨm ). Using murine hepatoma 1c1c7 cells and a photosensitizing agent known to target mitochondria, we examined loss of
Sylvie Skalickova et al.
Frontiers in nutrition, 9, 838963-838963 (2022-03-15)
Peas are prospectively beneficial legumes in the human diet, and especially in a vegan and vegetarian diet, due to their high content of proteins and starch. Their frequent lack of appeal in human nutrition can be caused by their bloating

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service