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TRACRRNA05N

SygRNA™ Cas9 Synthetic tracrRNA

SygRNA™ SpCas9 tracrRNA

Synonym(s):

RNA scaffold

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Storage temp.:
−20°C
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packaging

pkg of 5 nmol × Tube (HPLC)

shipped in

ambient

storage temp.

−20°C

General description

The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.

Application

Functional Genomics/Transgenic Applications
  • Creation of gene knockouts
  • Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

HPLC-purified SygRNA™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.

Physical form

Dry

Preparation Note

Resuspend SygRNA™ Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

Other Notes

The SygRNA™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.

Learn more about SygRNA™ tracrRNA

Need custom tracrRNA ?

Legal Information

CRISPR Use License Agreement
SygRNA is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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