TRACRRNA05N
SygRNA™ Cas9 Synthetic tracrRNA
SygRNA™ SpCas9 tracrRNA
Synonym(s):
RNA scaffold
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
Storage temp.:
−20°C
packaging
pkg of 5 nmol × Tube (HPLC)
shipped in
ambient
storage temp.
−20°C
General description
The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.
Application
Functional Genomics/Transgenic Applications
- Creation of gene knockouts
- Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Features and Benefits
HPLC-purified SygRNA™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.
Physical form
Dry
Preparation Note
Resuspend SygRNA™ Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.
Other Notes
The SygRNA™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.
Learn more about SygRNA™ tracrRNA
Need custom tracrRNA ?
Learn more about SygRNA™ tracrRNA
Need custom tracrRNA ?
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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