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Merck
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TRUECD

TrueGel3D Crosslinker

CD cell-degradable crosslinker

Synonym(s):

Crosslinker

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About This Item

NACRES:
NA.52
UNSPSC Code:
12352207
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shipped in

dry ice

storage temp.

−20°C

General description

CD cell-degradable crosslinker is comprised of conjugated peptide along with polyethylene glycol and thiol (SH) groups. The thiol groups form stable thioether bond with thiol-reactive groups of the polymers (Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran) and results in a stable hydrogel.

The conjugated peptide has matrix metalloprotease (MMP)-cleavage sites (Pro-Leu-Gly-Leu-Trp-Ala), which allows matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9) to locally degrade the polymer network. It allows cells to spread and migrate within the gel if they express the indicated MMPs.

Application

TRUECD is used to crosslink PVA and dextran polymers to generate cell-degradable hydrogel

Features and Benefits

MMP degradable

Other Notes

TRUECD contains CD cell-degradable crosslinker lyophilized and water.

Disclaimer

Avoid prolong exposure of CD cell-degradable crosslinker to air


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Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

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Protocols

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Articles

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物(例如小鼠EHS肿瘤细胞)的水凝胶相比,TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

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Global Trade Item Number

SKUGTIN
TRUECD-600UL04061835516902
TRUECD-200UL04061835516896