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Merck
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TRUEDEXF

TrueGel3D Polymer

Modified for fast gelling (FAST-DEXTRAN),allow cell recovery

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

Key Documents

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shipped in

dry ice

storage temp.

−70°C

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General description

Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Features and Benefits

  • Contains dextrose polymer functionalized with maleimide groups for quick gelation
  • Degradable polymer

Other Notes

TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water

Disclaimer

Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
0001615178

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Articles

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

3D细胞培养水凝胶_细胞培养_TrueGel3D™水凝胶-默克生命科学

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物（例如小鼠EHS肿瘤细胞）的水凝胶相比，TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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