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TRUEPVAF

TrueGel3D Polymer

Name: TrueGel3D Polymer
Brand: SIGMA

modified for fast gelling (FAST-PVA)

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About This Item

NACRES:

NA.71

UNSPSC Code:

12352207

Biological source:

synthetic

Technique(s):

cell attachment: suitable

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Properties

biological source

synthetic

technique(s)

cell attachment: suitable

shipped in

dry ice

storage temp.

−70°C

Description

General description

PVA polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

Application

FAST-PVA polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels.

Features and Benefits

Contains synthetic polymer (PVA) functionalized with maleimide groups for quick gelation
Non degradable polymer

Other Notes

TRUEPVAF contains FAST-PVA solution in phosphate buffer, TrueGel3D thioglycerol and water.

Disclaimer

Avoid frequent freeze-thaw cycles and store FAST-PVA polymer in small portions.

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pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number

0001615176

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Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Articles

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

3D细胞培养水凝胶_细胞培养_TrueGel3D™水凝胶-默克生命科学

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物（例如小鼠EHS肿瘤细胞）的水凝胶相比，TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

Global Trade Item Number

SKU	GTIN
TRUEPVAF-170UL	04061835539352