TSTOP
ThermaStop™
increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR
Synonym(s):
Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
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About This Item
form
lyophilized powder
packaging
pkg of 2500 units, pkg of 500 units, pkg of One unit of ThermaStop™ is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
technique(s)
PCR: suitable
color
opaque
shipped in
ambient
storage temp.
2-8°C
General description
Application
Features and Benefits
- Adding ThermaStop™ to your PCR reactions improves sensitivity and total product yield
- Increases the product yield for downstream applications like genotyping, next-generation sequencing (NGS) sample preparation, Gibson assembly, diagnostics, and multiplex PCR
- It improves polymerase specificity, enhances PCR performance and quantitative data
- Prevents primer-dimer formation and non-specific products before PCR
- Enables stabilization of master mixes at room temperature before PCR for up to 36 hrs
- Rapidly inactivated during the first PCR cycle
- Rapidly re-activated upon cooling
Preparation Note
Legal Information
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
How to stop nonspecific bands and off target primers in PCR.
PCR additive added to master mix that stops nonspecific bands after cool down.
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.