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Merck
CN

TSTOPRT

Sigma-Aldrich

ThermaStop™-RT

increases RT-PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before RT-PCR

Synonym(s):

Multiplex RT-PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart

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About This Item

UNSPSC Code:
12352200

Key Documents

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form

lyophilized powder

packaging

pkg of 2500 units
pkg of 500 units

technique(s)

RT-PCR: suitable

color

opaque

shipped in

ambient

storage temp.

2-8°C

General description

ThermaStop™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.

Application

ThermaStop™-RT is suitable for gene expression quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.

Features and Benefits

  • It eliminates the non-specific product formation
  • Provides increased sensitivity and specificity
  • Provides higher yield and pure cDNA synthesis for conventional RT-PCR
  • It minimizes the primer-dimer formation
  • It utilizes the same cycling conditions as conventional Taq polymerase
  • Decreases the RNA secondary structure
  • Stable at low temperatures
  • Enzymes are inhibited at below 55°C
  • Compatible with several RT-PCR enzymes
  • Provides better multiplexing, efficient primer use
  • Better quantitative accuracy and monoplex and multiplex amplification sensitivity

Packaging

One unit of ThermaStop™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 uL and a temperature of 50 degrees C.

Preparation Note

Dissolve in Tris pH 8.3 buffer

Other Notes

RT-PCR, *Please note ThermaStop™-RT should not be used in combination with ThermaStop™ or ThermaGo™.

Legal Information

ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

ThermaGenix PCR 添加剂可降低背景并提高产量

ThermaStop™ 和 ThermaGo™ PCR 添加剂可抑制扩增前、扩增中和扩增后的错配，从而提高PCR的特异性和灵敏度。ThermaStop™-RT添加剂在一步法和两步法RT-PCR中可作为逆转录酶的热启动剂。

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

ThermaStop™ PCR Additive

PCR additive added to master mix that stops nonspecific bands after cool down.

ThermaStop™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

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