TSTOPRT
ThermaStop™-RT
increases RT-PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before RT-PCR
Synonym(s):
Multiplex RT-PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
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About This Item
UNSPSC Code:
12352200
form
lyophilized powder
packaging
pkg of 2500 units, pkg of 500 units
technique(s)
RT-PCR: suitable, whole genome amplification: suitable
color
opaque
shipped in
ambient
storage temp.
2-8°C
General description
ThermaStop™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature. ThermaStop-RT acts as a Hot-start which binds and inhibits DNA polymerase activity. It also renatures the DNA polymerase upon cooling and activates its functions at lower temperatures or post-amplification.
Application
ThermaStop™-RT is suitable for gene expression quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.
Features and Benefits
- It eliminates the non-specific product formation
- Provides increased sensitivity and specificity
- Provides higher yield and pure cDNA synthesis for conventional RT-PCR
- It minimizes the primer-dimer formation
- It utilizes the same cycling conditions as conventional Taq polymerase
- Decreases the RNA secondary structure
- Stable at low temperatures
- Enzymes are inhibited at below 55°C
- Compatible with several RT-PCR enzymes
- Provides better multiplexing, efficient primer use
- Better quantitative accuracy and monoplex and multiplex amplification sensitivity
Packaging
One unit of ThermaStop™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 uL and a temperature of 50 degrees C.
Preparation Note
Dissolve in Tris pH 8.3 buffer
Other Notes
RT-PCR, *Please note ThermaStop™-RT should not be used in combination with ThermaStop™ or ThermaGo™.
Legal Information
ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.
wgk
WGK 3
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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