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U5382

Ubiquitin human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder

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About This Item

CAS Number:
60267-61-0
UNSPSC Code:
41106500
NACRES:
NA.32
MDL number:
MFCD02100852
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (N-terminal FLAG® tagged)
Mol wt:
10 kDa
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biological source

human

Quality Segment

200

recombinant

expressed in E. coli (N-terminal FLAG® tagged)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

10 kDa

technique(s)

mass spectrometry (MS): suitable

solubility

0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless, 0.05 M Tris pH 7.5: ≥10 mg/mL

UniProt accession no.

P0CG47, P0CG48

storage temp.

−20°C

Gene Information

human ... UBB(7314), UBC(7316)

General description

Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved globular protein and has lysine in the surface. The C-terminal end comprises the Leu-Arg-Gly-Gly structural motif.

Application

Ubiquitin human has been used in mono-ubiquitination of yeast proliferating cell nuclear antigen (PCNA) and in in vitro ubiquitination assay of defective in mitotic arrest 1 (Dma1)
Ubiquitin human can be used as a standard for mass spectrometry.
Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.

Biochem/physiol Actions

Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.
Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.
Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders.

Packaging

Package size based on protein content

Preparation Note

Ubiquitin human can be dissolved in 0.05 M Tris-HCl at a concentration of 10.00 - 11.00 mg/ml to yield a clear to slightly hazy, colorless solution.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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