U5382
Ubiquitin human
≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (N-terminal FLAG® tagged)
Mol wt:
10 kDa
biological source
human
Quality Level
recombinant
expressed in E. coli (N-terminal FLAG® tagged)
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
10 kDa
technique(s)
mass spectrometry (MS): suitable
solubility
0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless, 0.05 M Tris pH 7.5: ≥10 mg/mL
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved globular protein and has lysine in the surface. The C-terminal end comprises the Leu-Arg-Gly-Gly structural motif.
Application
Ubiquitin human can be used as a standard for mass spectrometry.
Ubiquitin human has been used in mono-ubiquitination of yeast proliferating cell nuclear antigen (PCNA) and in in vitro ubiquitination assay of defective in mitotic arrest 1 (Dma1)
Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.
Biochem/physiol Actions
Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.
Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders.
Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.
Packaging
Package size based on protein content
Preparation Note
Ubiquitin human can be dissolved in 0.05 M Tris-HCl at a concentration of 10.00 - 11.00 mg/ml to yield a clear to slightly hazy, colorless solution.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Boris Macek et al.
Molecular & cellular proteomics : MCP, 5(5), 949-958 (2006-02-16)
Top-down proteomics, the analysis of intact proteins (instead of first digesting them to peptides), has the potential to become a powerful tool for mass spectrometric protein characterization. Requirements for extremely high mass resolution, accuracy, and ability to efficiently fragment large
The ubiquitin-proteasome pathway and the regulation of growth hormone receptor availability
Strous, Ger J and van Kerkhof, Peter
Molecular and Cellular Endocrinology, 143-151 (2002)
Ana Camara-Artigas et al.
Acta crystallographica. Section F, Structural biology communications, 72(Pt 1), 29-35 (2016-01-12)
Ubiquitin is a small globular protein that has a considerable number of lysine residues on its surface. This results in a high surface entropy that precludes the formation of crystal-packing interactions. To date, only a few structures of the native
The ubiquitin-proteasome pathway and the regulation of growth hormone receptor availability
Strous, Ger J and van Kerkhof, Peter
Molecular and cellular endocrinology, 143-151 (2002)
Regulation of NF-kappaB by ubiquitination
Chen J and Chen ZJ
Current Opinion in Immunology, 4-12 (2013)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| U5382-1MG | 04061826677285 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service