recombinant
expressed in Sf9 cells
Quality Segment
form
buffered aqueous glycerol solution
mol wt
96 kDa
concentration
≥0.20 mg/mL protein (Bradford), ≥51% (SDS-PAGE)
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
General description
Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (isoT), amino acids 2-858 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
The tumor suppressor gene P53 was shown to be a target of ubiquitin-specific protease 5.
Physical form
Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable