U6885
Uridine-5′-diphosphoglucose Dehydrogenase from Streptococcus pyogenes
recombinant, expressed in E. coli, Type VI, lyophilized powder
Synonym(s):
UDP-glucose:NAD+ 6-oxidoreductase
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About This Item
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
type
Type VI
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
~47 kDa
shipped in
dry ice
storage temp.
−70°C
Quality Level
Physical form
Lyophilized powder containing ~70% PBS and ~20% sorbitol.
Preparation Note
Animal component free
Other Notes
One unit will oxidize 1.0 μmole of UDP-glucose to UDP-glucuronic acid per minute at pH 8.7 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Simone Superina et al.
Developmental biology, 387(2), 154-166 (2014-01-28)
Growth factors and morphogens regulate embryonic patterning, cell fate specification, cell migration, and morphogenesis. The activity and behavior of these signaling molecules are regulated in the extracellular space through interactions with proteoglycans (Bernfield et al., 1999; Perrimon and Bernfield 2000;
Annastasia S Hyde et al.
The Journal of biological chemistry, 288(49), 35049-35057 (2013-10-23)
UDP-glucose dehydrogenase (UGDH) provides precursors for steroid elimination, hyaluronan production, and glycosaminoglycan synthesis. The wild-type UGDH enzyme purifies in a hexamer-dimer equilibrium and transiently undergoes dynamic motion that exposes the dimer-dimer interface during catalysis. In the current study we created
Haifeng Li et al.
Applied biochemistry and biotechnology, 178(6), 1141-1153 (2015-11-29)
UDPG pyrophosphatase (UGPase) plays an important role in carbohydrate metabolism, catalyzing a reversible production of uridine diphosphate glucose (UDPG) and pyrophosphate (PPi) from Glc-1-P and UTP. UGPase gene from Aureobasidium pullulans NRRL Y-12974 was cloned, overexpressed in Escherichia coli. The
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