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U7883

Monoclonal Anti-USP25 antibody produced in mouse

~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-USP21, Anti-Ubiquitin specific peptidase 25

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Monoclonal Anti-USP25 antibody produced in mouse, ~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

U25.7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~126 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25.

isotype

IgG2a

UniProt accession no.

Q9UHP3

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... USP25(29761)
mouse ... Usp25(30940)
rat ... Usp25(304150)

Application

Monoclonal Anti-USP25 antibody has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

Biochem/physiol Actions

USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
129K4800
108K4772

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Erik Meulmeester et al.
Molecular cell, 30(5), 610-619 (2008-06-10)
Vertebrates express two distinct families of SUMO proteins (SUMO1 and SUMO2/3) that serve distinct functions as posttranslational modifiers. Many proteins are modified specifically with SUMO1 or SUMO2/3, but the mechanisms for paralog selectivity are poorly understood. In a screen for
A Bosch-Comas et al.
Cellular and molecular life sciences : CMLS, 63(6), 723-734 (2006-02-28)
The biological functions of the more than one hundred genes coding for deubiquitinating enzymes in the human genome remain mostly unknown. The USP25 gene, located at 21q11.2, encodes three protein isoforms produced by alternative splicing. While two of the isoforms

Related Content

Data Sheet - U7883

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