V4014
Anti-V5-Cy3™ antibody, Mouse monoclonal
purified immunoglobulin, ~1 mg/mL, clone V5-10
Synonym(s):
Monoclonal Anti-V5
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About This Item
Conjugate:
CY3 conjugate
Clone:
V5-10, monoclonal
Application:
ICC
Citations:
6
biological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V5-10, monoclonal
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 2.0-4.0 μg/mL
shipped in
wet ice
storage temp.
2-8°C
General description
Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.
Application
Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Legal Information
Cy3 is a trademark of Cytiva
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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