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Merck
CN

W0876

White′s Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

NACRES:
NA.72
UNSPSC Code:
12352207
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form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Quality Level

General description

White′s Basal Salt Mixture is widely used in plant tissue culture.

Application

White′s Basal Salt Mixture has been used:
  • as a medium for the precultured spores of Prothalli of Adiantum capillus for the generation of protonemata
  • to promote germination and cotton cotyledon unfolding
  • as a solidified medium component for culturing A. capillus and as a component of selection medium for gametophyte selection after particle bombardment

Preparation Note

With the macro- and micronutrients as described by White (1968).

Media Formulation

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Ox. Sol. 3 - STOT RE 2 Inhalation

target_organs

Brain

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Hiroko Kawai-Toyooka et al.
Plant & cell physiology, 45(11), 1648-1657 (2004-12-03)
RNA interference (RNAi) has become a powerful tool for determining gene function and is used in a wide variety of organisms. Since it is necessary to generate double-stranded RNA (dsRNA) as an inducer for RNAi, preparation of RNAi-inducing constructs is
White's standard nutrient solution
SINGH M and Krikorian AD
Annals of Botany, 47, 133-139 (1981)
Kagawa et al.
Plant physiology, 119(3), 917-924 (1999-03-09)
Chloroplast movement was induced by partial cell illumination using a high-fluence blue microbeam in light-grown and dark-adapted prothallial cells of the fern Adiantum capillus-veneris. Chloroplasts inside the illuminated area moved out (high-fluence response [HFR]), whereas those outside moved toward the
W Bressan et al.
Canadian journal of microbiology, 46(8), 741-743 (2000-08-15)
Responses of somatic embryos of sweet potato (Ipomoea batata (L.) Poir., cv. White Star) at different developmental stages to in vitro inoculation with Glomus etunicatum (Becker and Gerdemann) (isolate INVAM FL329) were evaluated. Somatic embryos were grown in glass tubes
S J Sata et al.
Methods in cell science : an official journal of the Society for In Vitro Biology, 22(4), 299-304 (2001-09-11)
An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was

Articles

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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