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X4376

Xanthine Oxidase from bovine milk

lyophilized powder, 0.4-1.0 units/mg protein

Synonym(s):

XOD, Xanthine:oxygen oxidoreductase

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About This Item

CAS Number:
9002-17-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-657-6
MDL number:
MFCD00082145
EC Number:
1.17.3.2.
Specific activity:
0.4-1.0 units/mg protein
Biological source:
bovine milk
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biological source

bovine milk

Quality Segment

form

lyophilized powder

specific activity

0.4-1.0 units/mg protein

mol wt

283 kDa

composition

Protein, 10.0-25.0%

storage temp.

2-8°C

General description

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used as a source for superoxide generation. It has also been used as a model enzyme in the internally calibrated electrochemical continuous enzyme assay (ICECEA).

Biochem/physiol Actions

Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.
The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Physical form

Contains 0.5% sodium salicylate.

Analysis Note

Protein determined by biuret

Other Notes

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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