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X4626

XTT sodium salt

Name: XTT sodium salt
Brand: SIGMA

powder, BioReagent, suitable for cell culture

Synonym(s):

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₆N₇NaO₁₃S₂

CAS Number:

111072-31-2

Molecular Weight:

673.52

NACRES:

NA.75

PubChem Substance ID:

24902150

UNSPSC Code:

12352207

MDL number:

MFCD00083517

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product line

BioReagent

Quality Level

200

assay

≥90%

form

powder

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable, protein quantification: suitable

application(s)

detection

detection method

colorimetric

storage temp.

2-8°C

SMILES string

[Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1

InChI key

JACYMBNQPPWQML-UHFFFAOYSA-M

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General description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Application

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Biochem/physiol Actions

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

pictograms

GHS02

signalword

Danger

hcodes

H242

pcodes

P210 - P234 - P235 - P240 - P370 + P378 - P403

Hazard Classifications

Self-react. C

supp_hazards

EUH044

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Reduced tissue and serum resistin expression as a clinical marker for esophageal squamous cell carcinoma.

Amos C Hung et al.

Oncology letters, 22(5), 774-774 (2021-10-01)

Esophageal cancer is one of the most common malignancies and leading cause of cancer-associated mortality worldwide. However, the molecular mechanisms underlying esophageal cancer progression and the development of clinical tools for effective diagnosis remain unclear. Resistin, which was originally identified

Antimicrobial activity, cytotoxicity and chemical analysis of lemongrass essential oil (Cymbopogon flexuosus) and pure citral

Adukwu EC, et al.

Applied Microbiology and Biotechnology, 100(22), 9619-9627 (2016)

Improved short- and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells.

L M Jost et al.

Journal of immunological methods, 147(2), 153-165 (1992-03-04)

A tetrazolium compound, XTT, bioreducible to a water-soluble formazan was used to develop a simplified cellular cytotoxicity assay. Most (13/15 melanoma and 2/3 colon carcinoma cell lines tested metabolized XTT greater than 50 times more efficiently than the lymphoid effector

Metal-Organic Frameworks as Drug Delivery Platforms for Ocular Therapeutics.

Jesús Gandara-Loe et al.

ACS applied materials & interfaces, 11(2), 1924-1931 (2018-12-19)

Metal-organic frameworks (MOFs) have been evaluated as potential nanocarriers for intraocular incorporation of brimonidine tartrate to treat chronic glaucoma. Experimental results show that UiO-67 and MIL-100 (Fe) exhibit the highest loading capacity with values up to 50-60 wt %, whereas

An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT.

N W Roehm et al.

Journal of immunological methods, 142(2), 257-265 (1991-09-13)

A new tetrazolium salt XTT, sodium 3'-[1-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6- nitro)benzene-sulfonic acid hydrate, was evaluated for use in a colorimetric assay for cell viability and proliferation by normal activated T cells and several cytokine dependent cell lines. Cleavage of XTT by dehydrogenase enzymes

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Global Trade Item Number

SKU	GTIN
X4626-500MG	04061835514250
X4626-100MG	04061835514243

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