XNAB2R
Extract-N-Amp™ Blood PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonym(s):
Blood direct PCR kit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
Quality Segment
usage
sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable
color
colorless
input
crude DNA
shipped in
wet ice
storage temp.
−20°C
General description
The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, that uses an antibody based hot start and is specially formulated for amplification directly from the extract.
Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.
Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.
The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
Application
Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).
Biochem/physiol Actions
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
Features and Benefits
- Efficient 8 minute prep allows greater speed and throughput
- No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
- Simple, 3 step procedure with no special equipment required
- Hot start antibody included for highly specific PCR amplification of genomic DNA
- Compatible with multiple format (single tube or 96-well plate)
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
- Efficient 8 minute prep allows greater speed and throughput
- No need for organic extraction, centrifugation or alcohol precipitation
- Hot start antibody for highly specific PCR amplification of genomic DNA
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Still not finding the right product?
Explore all of our products under Extract-N-Amp™ Blood PCR Kit
Storage Class
10 - Combustible liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| XNAB2R-1KT | 04061837549885 |