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XNACRL

Extract-N-Amp™ Cellular RNA Lysis Buffer

Optimized for rapid cell lysis and RNA stabilization

Sustainability features

Synonym(s):

RNA extraction buffer, RNA isolation buffer, RNA lysis buffer

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About This Item

UNSPSC Code:
41106306
NACRES:
NA.25
eCl@ss:
32039090
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Quality Segment

form

liquid

feature

Direct PCR

storage condition

OK to freeze (-10 to-25°C)

sustainability

Sustainability features

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

suitability

suitable for PCR (suitable for RT-qPCR)

greener alternative category

shipped in

dry ice

storage temp.

−20°C

General description

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

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Application

  • The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
  • qRT-PCR
  • RT-LAMP
  • NGS library preparation

Features and Benefits

  • From cell lysis to qRT-PCR in 15 minutes
  • High Throughput and automation-friendly
  • No RNA purification or clean up necessary
  • No columns, heat, or centrifugation necessary
  • Compatible with both adherent and non-adherent mammalian cell types
  • Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

Physical form

Liquid

Preparation Note

Freezer (-10 to-25°C)

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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