Y1003
2x YT medium
Liquid microbial growth medium
Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106225
grade
Molecular Biology
Quality Segment
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.5
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl
10 g/L Yeast Extract
5.0 g/L NaCl
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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