Y1003
2x YT medium
Liquid microbial growth medium
Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106225
grade
Molecular Biology
Quality Level
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.5
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl
10 g/L Yeast Extract
5.0 g/L NaCl
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
动物来源培养基
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Victor V Kuprianov et al.
Virus research, 284, 197984-197984 (2020-04-24)
Hepatitis C virus (HCV) can cause chronic infection and evade the immune response. The generation and maintenance of an effective T-cell response is important for immune-mediated control of HCV infection. The purpose of this study was to obtain recombinant mosaic
Dynamical model fitting to a synthetic positive feedback circuit in E. coli
Tica J. et al.
Journal of Separation Science (2020)
Nicholas Collins et al.
Cell, 178(5), 1088-1101 (2019-08-24)
Mammals evolved in the face of fluctuating food availability. How the immune system adapts to transient nutritional stress remains poorly understood. Here, we show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y1003-500ML | 04061837587818 |