Yeast Synthetic Drop-out Medium Supplements

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

• the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
• Yarrowia lipolytica PO1f strain positive transformants
• CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

Dissolve 1.46 g of supplement per liter of medium.

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.