Key Documents

View All Documentation

Y2377

2x YT medium

Name: 2x YT medium
Brand: SIGMA

Powder microbial growth medium

Synonym(s):

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media

Select a Size

Change View

About This Item

NACRES:

NA.85

UNSPSC Code:

41106225

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.1% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Looking for similar products? Visit Product Comparison Guide

Related Categories

Cloning & Expression

Industrial Microbiology

Microbial Culture Media

Molecular Cloning & Protein Expression

General description

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Features and Benefits

2xYT powder provides:

Standard formulation
An inexpensive alternative to liquid

Preparation Note

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Designed active-site library reveals thousands of functional GFP variants

Weinstein J Y. et al.

Nature Communications (2023)

Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants

Graham ND, et al.

Bio-protocol, 5(18) (2015)

Bacterial artificial chromosomes: A functional genomics tool for the study of positive-strand RNA viruses

Yun SI, et al.

Journal of Visualized Experiments, 106, e53164-e53164 (2015)

Innate Immune Stimulation Using 3D Wireframe DNA Origami.

Rebecca R Du et al.

ACS nano, 16(12), 20340-20352 (2022-12-03)

Three-dimensional wireframe DNA origami have programmable structural and sequence features that render them potentially suitable for prophylactic and therapeutic applications. However, their innate immunological properties, which stem from parameters including geometric shape and cytosine-phosphate-guanine dinucleotide (CpG) content, remain largely unknown.

Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors.

Basem Al-Shayeb et al.

Cell, 185(24), 4574-4586 (2022-11-25)

CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Global Trade Item Number

SKU	GTIN
Y2377-250G	04061837588020

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service