Z-2018
ZytoLight® SPEC FOXO1/PAX3 Dual Color Single Fusion Probe
orange to green, pkg of 0.2 mL, pkg of 0.05 mL
Synonym(s):
FISH probe, dual color FISH probe
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packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
orange to green
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC FOXO1/PAX3 Dual Color Single Fusion Probe is a direct-labeled FISH probe designed for qualitative detection of the t(2;13)(q36;q14.1) translocation of the FOXO1 and PAX3 genes in formalin-fixed, paraffin-embedded specimens, using fluorescence in situ hybridization (FISH). It is intended for use with the ZytoLight FISH-Tissue Implementation Kit.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FOXO1/PAX3 Dual Color Single Fusion Probe comprises:
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
ZytoLight® probes enable the precise identification of genetic abnormalities, including translocations, deletions, amplifications, and chromosomal aneuploidies, in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens, without the need for additional antibody-based detection.
Components
The ZytoLight SPEC FOXO1/PAX3 Dual Color Single Fusion Probe comprises:
- ZyOrange (excitation 547 nm/emission 572 nm) labeled polynucleotides (~4.5 ng/µl), targeting sequences mapping in 13q14.11* (chr13:40,816,168-41,132,595) proximal to the FOXO1 breakpoint region;
- ZyGreen (excitation 503 nm/emission 528 nm) labeled polynucleotides (~10.0 ng/µl), targeting sequences mapping in 2q36.1* (chr2:223,196,078-223,539,352) distal to the PAX3 breakpoint region;
- Formamide based hybridization buffer
Principle
The fluorescence in situ hybridization (FISH) method enables the identification and visualization of particular nucleic acid sequences within cell samples. DNA fragments labeled with fluorescent dyes, known as FISH probes, are co-denatured with their complementary target DNA strands in the sample and then allowed to hybridize. Following hybridization, non-specific and unbound probe fragments are removed through stringency washes. The DNA is then counterstained with DAPI, and the hybridized probes are detected using a fluorescence microscope equipped with filters matched to the specific fluorochromes used to label the FISH probes.
Application
This probe is intended for use in formalin-fixed, paraffin-embedded specimens to qualitatively detect t(2;13)(q36;q14.1) translocation involving the FOXO1 and PAX3 genes, via FISH.
Analysis Note
Using suitable filter sets, the hybridization signals of the probe appear orange near the FOXO1 breakpoint region, and green away from the PAX3 breakpoint region.
- Normal scenario: in normal cells during interphase or cells without a translocation of the respective gene regions, two separate green and orange signals are observed.
- Aberrant scenario: gene fusion is indicated by a separate orange signal, a separate green signal, and a orange/green fusion signal.
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Target Organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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