ZytoLight^® SPEC MDM4/1p12 Dual Color Probe

The ZytoLight^® SPEC MDM4/1p12 Dual Color Probe (PL39, Z-2080-200) is a FISH probe designed for the qualitative detection of amplifications involving the human MDM4 gene and specific sequences on chromosome 1p12 in formalin-fixed, paraffin-embedded (FFPE) specimens. This probe utilizes ZyGreen-labeled polynucleotides to detect MDM4 gene amplifications at 1q32.1 (chr1:204,126,022-204,882,307, 10 ng/µl) and ZyOrange-labeled polynucleotides for the 1p12 reference sequences (chr1:119,537,102-119,823,147, 4.5 ng/µl). It is intended for professional use only in certified, licensed anatomic pathology laboratories under the supervision of qualified personnel and is to be used in conjunction with the ZytoLight FISH-Tissue Implementation Kit (Prod. No. Z-2028-5/-20).

Principle:

The ZytoLight^® system employs fluorescence in situ hybridization (FISH) to detect and visualize specific nucleic acid sequences in formalin-fixed, paraffin-embedded (FFPE) specimens. Direct-labeled probes co-denature with their complementary target DNA strands, enabling hybridization. Unbound probes are washed away, and hybridized fragments are counterstained with DAPI.The ZytoLight^® SPEC MDM4/1p12 Dual Color Probe uses:ZyGreen: Targets the MDM4 gene at 1q32.1 (excitation 503 nm/emission 528 nm).ZyOrange: Targets sequences at 1p12 (excitation 547 nm/emission 572 nm).FISH visualizes green signals for MDM4 and orange signals for 1p12, using a microscopy setup that includes an exciter filter, a dichroic mirror, and a barrier filter. This system is optimized for identifying amplifications and aneuploidies in FFPE samples.

Components:

Probe Composition:

• ZyGreen: Labeled polynucleotides (~10 ng/µl) targeting the MDM4 gene region at 1q32.1 (chr1:204,126,022-204,882,307).
• ZyOrange: Labeled polynucleotides (~4.5 ng/µl) targeting sequences at 1p12 (chr1:119,537,102-119,823,147).
• Hybridization Buffer: Formamide-based hybridization buffer.

Product Size:- Z-2080-200: 0.2 ml (20 reactions of 10 µl each).- Related Products: Intended for use with the ZytoLight FISH-Tissue Implementation Kit (Prod. No. Z-2028-5/-20).

ZytoLight^® SPEC MDM4/1p12 Dual Color Probe aids differential diagnosis of various cancers via MDM4 (p53 regulator, aka HDMX/MDMX) amplification detection; MDM4 inhibits p53 like MDM2, relevant for antitumor therapies targeting both.

For the qualitative detection of human MDM4 gene amplifications and chromosome 1p12 specific sequences by fluorescence in situ hybridization (FISH)

In normal interphase nuclei, the expected results are two green signals (for the MDM4 gene) and two orange signals (for the 1p12 locus). Cells with amplification of the MDM4 gene or aneuploidy of chromosome 1 will exhibit multiple copies of the green signal or large green signal clusters. Variations in signal patterns may indicate different genetic rearrangements and should be investigated further.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany