ZytoLight^® SPEC RREB1/MYB/CEN 6 Triple Color Probe

The ZytoLight SPEC RREB1/MYB/CEN 6 Triple Color Probe (PL108) is a direct-labeled FISH probe designed for the qualitative detection of amplifications involving the human RREB1 gene, the human MYB gene, and chromosome 6 alpha satellites in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used in conjunction with the ZytoLight FISH-Tissue Implementation Kit.

Components:

ZytoLight® SPEC RREB1/MYB/CEN 6 Triple Color Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm), targeting the RREB1 gene region on chromosome 6 (6p24.3).
• ZyRed labeled polynucleotides (excitation 580 nm/emission 599 nm), targeting the MYB gene region on chromosome 6 (6q23.3).
• ZyBlue labeled polynucleotides (excitation 418 nm/emission 467 nm), targeting the alpha satellite centromeric region D6Z1 of chromosome 6.
• Formamide-based hybridization buffer.

Principle:

Fluorescence in situ hybridization (FISH) uses direct-labeled probes (RREB1, MYB, CEN 6) that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.

The ZytoLight SPEC RREB1/MYB/CEN 6 Triple Color Probe is intended for the qualitative detection of amplifications involving RREB1 and MYB, and for detection of chromosome 6 alpha satellites, in the specified tissue context. It is designed to be used with the ZytoLight FISH-Tissue Implementation Kit.

In a normal interphase nucleus, two green signals (for RREB1), two red signals (for MYB), and two blue signals (for chromosome 6) are expected. In cases of amplification, an increased number of green or red signals may be observed. Any unexpected signal patterns should be further explored to identify potential genetic rearrangements.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany