ZytoLight^® SPEC NTRK1 Dual Color Break Apart Probe

The ZytoLight SPEC NTRK1 Dual Color Break Apart Probe (PL123) is a direct-labeled FISH probe designed for the qualitative detection of translocations involving the human NTRK1 gene at 1q23.1 in formalin-fixed, paraffin-embedded (FFPE) specimens. It should be used in conjunction with the ZytoLight FISH-Tissue Implementation Kit.

Components:


ZytoLight® SPEC NTRK1 Dual Color Break Apart Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting sequences distal to the NTRK1 breakpoint region.
• ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting sequences proximal to the NTRK1 breakpoint region.
• Formamide-based hybridization buffer.

Principle:

Fluorescence in situ hybridization (FISH) uses direct-labeled probes flanking NTRK1 breakpoint that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.

For the qualitative detection of translocations involving the human NTRK1 gene at 1q23.1 by fluorescence in situ hybridization (FISH)

In a normal interphase nucleus, two distinct signals corresponding to the NTRK1 gene are expected. In cases of translocation, aberrant signal patterns may indicate genetic rearrangements that warrant further investigation.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany