Anti-Cyclin E2 Antibody, clone 1F10 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

G1/S-specific cyclin-E2 (UniProt: O96020) is encoded by the CCNE2 gene (Gene ID: 9134) in human. The G1/S-specific cyclin-E2 (cyclin-E2) is a critical regulator of the cell cycle and has garnered significant attention due to its roles in cell proliferation, DNA replication, and its implications in oncogenesis. Cyclin-E2 is highly homologous to cyclin-E1, with approximately 50% sequence identity, and contains conserved domains such as the cyclin box, nuclear localization signals (NLS), and centrosome localization signals (CLS). Cyclin-E2 interacts with cyclin-dependent kinase 2 (CDK2), forming a kinase complex that drives the G1/S phase transition by phosphorylating substrates like retinoblastoma protein (Rb), E2F transcription factors, and other components of the pre-replication complex (PRC). Cyclin-E2 expression peaks during the G1/S transition, regulated predominantly by E2F transcription factors, which are released from Rb upon phosphorylation by CDK4/6-cyclin-D complexes. Cyclin-E2/CDK2 activity modulates critical cellular processes, including initiation of DNA replication, centrosome duplication, and histone biosynthesis. Unlike cyclin-E1, cyclin-E2 is implicated in distinct regulatory functions, including interactions with centrosomic proteins and potential roles in cellular stress response pathways. Cyclin-E2 localizes to the nucleus during late G1 and early S-phase, aligning with its role in transcriptional regulation and DNA replication initiation. Cyclin-E2 degradation is mediated by the SCF-FBW7 ubiquitin ligase complex, ensuring tight regulation throughout the cell cycle. Dysregulation of cyclin-E2, often through gene amplification or FBXW7 mutations, is frequently observed in cancers, contributing to replication stress, genomic instability, and resistance to therapies. Elevated cyclin-E2/CDK2 activity correlates with poor prognosis across multiple tumor types. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human Cyclin E2.

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cyclin E2 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cyclin E2 in L6 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound with Cyclin E2 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Cyclin E2 from Jurkat cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 1F10 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Cyclin E2. It targets an epitope within 23 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.