Anti-GATA1 Antibody, clone 1D3 ZooMAb^® Rabbit Monoclonal

Erythroid transcription factor (GATA1) (UniProt: P15976; also known as Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein) is encoded by the GATA1 (also known as ERYF1, GF1) gene (Gene ID: 2623) in human. It belongs to the GATA family of proteins. They act as tissue specific master transcriptional regulators. GATA1 binds to the consensus DNA sequence 5′-[A/T]GATA[A/G]-3′ in the regulatory regions of certain genes and plays a pivotal role in the regulation of erythropoiesis and megakaryopoiesis. The protein contains an N-terminal and a C-terminal Cys4-type zinc finger domain and a transactivation domain. GATA1 interacts with various co-factors and proteins to regulate gene expression. It forms complexes with other transcription factors, chromatin modifiers, and transcriptional co-activators and co-repressors. These interactions contribute to the precise control of erythroid-specific gene expression programs. The GATA1 gene is located on the X chromosome. Mutations in the gene have been associated with several disorders, including Diamond-Blackfan anemia, X-linked thrombocytopenia, transient abnormal myelopoiesis and myeloid leukemia of Down syndrome. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.:, Crispino, J.D. and Horwitz, M.S., (2017) Blood 129(15), 2103-10)

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of Human GATA1.

Quality Control Testing

Evaluated by Western Blotting with His/T7 tagged, recombinant fragment of Mouse GATA1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His/T7 tagged, recombinant fragment of Mouse GATA1.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GATA1 in K-562 cell nuclear extract.

Affinity Binding Assay: A representative lot of this antibody bound GATA1 peptide with a KD of 2.8 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GATA1 in K-562 cells.

Enzyme Immunoassay (ELISA): Serial dilutions from a representative lot detected His/T7 tagged, recombinant fragment of Mouse GATA1.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D3 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Human GATA1. It targets an epitope within 22 amino acids from the N-terminal half of Human GATA1.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.