Anti-Notch 3 Antibody, clone 1G17 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1G17 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Notch 3. It targets an epitope within 17 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Notch 3.

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Notch 3 in K562 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Notch 3 in MCF-7, HepG2, and Neuro2a cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound Notch 3 peptide with a KD of 2.2 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Notch 3 in MCF-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Notch 3 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Neurogenic locus notch homolog protein 3 (UniProt: Q9UM47; also known as Notch 3) is encoded by the NOTCH3 gene (Gene ID: 4854) in human. Notch 3 is a single-pass, type I membrane protein that is synthesized with a signal peptide (aa 1-39), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 40-1643), a transmembrane domain (aa 1644-1664), and a cytoplasmic domain (aa 1665-2321). It is ubiquitously expressed in fetal and adult tissues. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Notch3 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in the formation of a C-terminal fragment and an N-terminal fragment. Mature Notch3 assembles as a heterodimeric protein. Binding of ligand triggers heterodimer dissociation that exposes proteolytic cleavage site (aa 1571-1572) resulting in the release of the Notch intracellular domain (NICD) that translocates to the nucleus and activates transcription of downstream target genes. Its N-terminal half contains 34 EGF-like domains, of which domains 10 and 11 are required for binding to its ligands. The C-terminal half contains 5 Ankyrin repeats. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Pathogenic mutations predominantly affect cysteine residues within EGF-like repeats in the extracellular domain. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Peters, N., et al. (2004). EXP^®. Cell Res. 299(2); 454-464; Arboleda-Velasquez, JF., et al. (2002). Neurology. 59(2); 277-279; Dichgans, M., et al. (2000). Eur. J. Hum. Genet. 8(4); 280-285).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Affinity is a trademark of Mine Safety Appliances Co.

EXP is a registered trademark of Optimize Technologies, Inc.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.