Anti-SERINC5 Antibody, clone 2C10 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Serine incorporator 5 (UniProt:Q86VE9) is encoded by the SERINC5 (also known as C5orf12 ) gene (Gene ID: 256987) in human. SERINC5 is a member of the serine incorporator family, is characterized by its role as a multipass transmembrane protein involved in lipid metabolism and antiviral defense. Its a non-ATP-dependent, non-specific lipid transporter for phosphatidylserine, phosphatidylcholine, and phosphatidylethanolamine. It also functions as a scramblase that flips lipids in both directions across the membrane. Structurally, SERINC5 contains approximately 10 to 11 transmembrane domains, enabling its localization predominantly at the plasma membrane, where it plays a critical role in inhibiting viral entry by disrupting the fusion of retroviral particles with host cells. SERINC5 is highly expressed in various tissues including placenta, skeletal muscle, spleen, thymus, testis and peripheral leukocyte and, is known to be weakly expressed in the heart, liver and fetal brain. Four isoforms of SERINC5 have been described that are produced by alternative splicing. Its involvement in disease is particularly significant in the context of HIV-1 infection, where the viral protein Nef counteracts SERINC5′s antiviral effects by redirecting it to lysosomes for degradation, thereby enhancing viral infectivity. Furthermore, mutations and dysregulation of SERINC5 have been associated with several pathologies, including neurodegenerative diseases and certain cancers. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human SERINC5.

Quality Control Testing
Evaluated by Western Blotting in A-431 cell lysate. 

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SERINC5 in A-431 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SERINC5 in A-431 cells.
Affinity Binding Assay: A representative lot of this antibody bound SERINC5 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SERINC5 in human prostate tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2C10 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects SERINC5. It targets an epitope within 25 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.