Anti-PGK1 Antibody, clone 2B12 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Phosphoglycerate kinase 1 (UniProt: P00558; also known as Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, EC:2.7.11.1, EC:2.7.2.3) is encoded by the PGK1 (also known as PGKA) gene (Gene ID: 5230) in human. A pseudogene of PGK1 gene has been found on the X-chromosome and another on chromosome 19 and 21. Structurally, PGK1 is characterized by a highly conserved domain architecture, facilitating its function as both a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and, a protein kinase when localized to mitochondria, where it phosphorylates pyruvate dehydrogenase kinase 1, thereby regulating cellular metabolism and promoting glycolysis over oxidative phosphorylation. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The protein may also act as a cofactor for polymerase alpha. PGK1 exhibits a ubiquitous expression profile across various tissues. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. Two isoforms of PGK1 have been described that are produced by alternative splicing. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Dysregulation of PGK1 is implicated in several diseases, including phosphoglycerate kinase deficiency, which manifests as hemolytic anemia and neurological impairments due to mutations affecting PGK1 expression and function. Studies suggested that activation of Pgk1 in cytosol may lead to a reduction in protein aggregation across various neurodegenerative conditions. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, H., et al. (2023). Mol. Neurobiol. 60(9):5090-5101).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human PGK1.

Quality Control Testing
Evaluated by Western Blotting in U-937 cell lysate. 
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PGK1 in U-937 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected PGK1 in lysate from HT-29 cells.
Affinity Binding Assay: A representative lot of this antibody bound PGK1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PGK1 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2B12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects PGK1. It targets an epitope within 19 amino acids from the Internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.