Anti-BTK Antibody, clone 1F5 ZooMAb^® Rabbit Monoclonal

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Tyrosine-protein kinase BTK (UniProt: Q06187; also known as EC:2.7.10.2, Agammaglobulinemia tyrosine kinase (ATK), B-cell progenitor kinase (BPK), Bruton tyrosine kinase, BTK) is encoded by the BTK (also known as AGMX1, ATK, BPK) gene (Gene ID: 695) in human. BTK is a non-receptor tyrosine kinase that is predominantly expressed in B-lymphocytes where it is involved in B cell development, differentiation, and signaling. In steady state, it is predominantly localized in the cytoplasm and following B-cell receptor (BCR) engagement by antigen, it translocates to the plasma membrane via its PH domain (aa 3-133). Its plasma membrane localization is reported to be essential for its activation. It can also shuttle between the nucleus and the cytoplasm, and its nuclear export is mediated by CRM1. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. It also plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway that links TLR8 and TLR9 to NF- B signaling pathway. BTK is activated following its phosphorylation. In primary B cells it is almost always detected in non-phosphorylated, catalytically inactive state. However, following BCR engagement and translocation to the plasma membrane, where it gets phosphorylated at tyrosine 551 by LYN and SYK, which is then followed by autophosphorylation of tyrosine 223 residue that may create a docking site for a SH2 containing proteins. Phosphorylation at serine 180 by PKC-β leads in its translocation back to cytoplasm. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mohamed, AJ., et al. (2009). Immunol. Rev. 228(1); 58-73; Horwood, NJ., et al. (2006). J. Immunol. 176(6); 3635-3641).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human Bruton tyrosine kinase (BTK).

Quality Control Testing

Evaluated by Western Blotting with His-tagged, full-length, recombinant, Human BTK.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, full-length, recombinant, Human BTK.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected BTK in lysates from Ramos cells and Rat spleen tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BTK in Ramos cells.

Affinity Binding Assay: A representative lot of this antibody bound BTK peptide with a KD of 2.9 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: Serial dilutions from a representative lot detected His-tagged, full-length, recombinant, Human BTK in ELISA.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1F5 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Bruton tyrosine kinase (BTK). It targets an epitope within 24 amino acids from the internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.