Anti-NUCB1 Antibody, clone 1F16 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Nucleobindin-1 (UniProt: Q02818; also known as NUC) is encoded by the NUCB1 (also known as CALNUC) gene (Gene ID: 4924) in human. Nucleobindin-1 (NUCB1) is a multifunctional calcium-binding protein predominantly localized in the Golgi apparatus. NUCB1 contains EF-hand motifs that undergo conformational changes upon calcium binding, facilitating protein-protein and nucleic acid interactions. It is also characterized by a signal peptide and a leucine zipper domain, which are critical for its function and localization. NUCB1 acts as a major calcium-binding protein involved in calcium homeostasis and intracellular signaling. NUCB1 serves as a non-receptor guanine nucleotide exchange factor (GEF), activating G-protein alpha subunits, which are essential for various signaling pathways. It plays a role in regulating the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress by modulating the cleavage of ATF6, a key transcription factor in UPR signaling. NUCB1 has also been implicated in maintaining Golgi structure and function, as well as in neuronal processes, where it acts as a marker for neuronal cells. The expression of NUCB1 is observed across diverse tissue types, with enriched profiles in neuronal and immune-related cells. Dysregulation of NUCB1 has been associated with several diseases, including prediabetes syndrome and kidney renal clear cell carcinoma, where it is considered a prognostic marker. Its role in calcium signaling and cellular stress responses links NUCB1 to pathological conditions such as cancer and metabolic disorders. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human NUCB1.

Quality Control Testing
Evaluated by Western Blotting in PC-3 cell lysate.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected NUCB1 in PC-3 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 Dilution from a representative lot detected NUCB1 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound with NUCB1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution from a representative lot detected NUCB1 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone 1F16 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects NUCB1. It targets an epitope within 20 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.