Anti-PPP1A Antibody, clone 1H8 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (UniProt: P62136; also known as EC:3.1.3.16, PP-1A) is encoded by the PPP1CA (also known as PPP1A) gene (Gene ID: 5499) in human. PPP1A is one of the three catalytic subunits of protein phosphatase 1 (PP1), a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. In addition to PP1 alpha, there is PP1 beta (PPP1CB), and two splice variants of PP1 gamma (PPP1CC). PPP1A interacts with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate a huge variety of target proteins. PPP1A is critical for cell division, chromatin structure, protein synthesis, glycogen metabolism, muscle and cardiac function as well as the regulation of long-term ionic conductances and even synaptic plasticity. Developmentally, it has nearly as many roles with principal ones being as a regulator of neural tube and optic fissure closure as well as enteric neural crest cell (ENCCs) migration during development. PPP1A protein is ubiquitously expressed in human tissues, with particularly high levels in the brain, heart, and skeletal muscle, reflecting its fundamental role in cellular signaling pathways. PPP1A can be found both cytoplasmically and nuclear localized depending upon its regulatory partner but PPP1A is mostly nuclear/nucleoplasm localized. Three isoforms of PPP1A have been described that are produced by alternative splicing. Dysregulation of PPP1A has been implicated in several diseases, including cancer, where altered phosphatase activity can lead to aberrant cell growth and survival. PPP1A has been linked to arthritis, playing distinct roles in both inflammatory and degenerative forms of the disease. It is suggested to be up-regulated in synovial fluid mononuclear cells and peripheral blood mononuclear cells from patients with rheumatoid arthritis. In the brain, PPP1A also functions as a suppressor of learning and memory and, as a potential mediator of cognitive decline during aging. It has also been associated Alzheimer′s disease. Recent studies suggest crucial role of PPP1CA in regulating HCC tumorigenesis through a YAP-dependent mechanism and identify it as a promising therapeutic target for HCC intervention. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, X., et al. (2025). Cell. Signal. 134; 111938).

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human PPP1A.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PPP1A in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PPP1A in lysates from A-431cells, C6 cells, NIH/3T3 cells, human kidney tissue, and mouse kidney tissue.

Affinity Binding Assay: A representative lot of this antibody bound PPP1A peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PPP1A in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1H8 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects PPP1A. It targets an epitope within 15 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.