Anti-ATF1 Antibody, clone 1N21 ZooMAb^® Rabbit Monoclonal

Cyclic AMP-dependent transcription factor ATF-1 (UniProt: P18846; also known as cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36) is encoded by the ATF1 gene (Gene ID: 466) in human. Cyclic AMP-dependent transcription factor ATF-1, member of the ATF subfamily within the basic-region leucine zipper (bZIP) family of transcription factors. This protein is activated via cAMP-dependent protein kinase A (PKA) signaling, enabling it to bind cyclic AMP response elements (CREs) on target DNA sequences and regulate gene transcription. ATF-1 contains a bZIP domain, which mediates dimerization and DNA binding. The protein binds CREs with the consensus sequence 5′-GTGACGT[AC][AG]-3′, influencing transcriptional activity in response to cellular signals such as cAMP. Its expression is ubiquitous, with variations across tissues depending on cellular activation states. ATF-1 interacts with other transcription factors, including CREB and ATF2, forming dimers to modulate transcriptional activity. It also interacts with kinases such as HIPK2, which phosphorylate ATF-1 in response to genotoxic stress. ATF-1 is implicated in multiple signaling pathways, including cAMP/PKA signaling, activating CRE-dependent genes during stress or hormonal stimulation and Toll-like receptor signaling, contributing to immune responses. Mutations or dysregulation of ATF-1 activity are associated with diseases such as melanoma of soft tissue and soft tissue sarcomas. Chromosomal translocations involving ATF1 (e.g., with EWSR1) result in chimeric proteins linked to angiomatoid fibrous histiocytoma and clear cell sarcoma. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal of human ATF1.

Quality Control Testing
Evaluated by Western Blotting in nuclear extract from HEK-293 cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ATF1 in nuclear extract from HEK-293 cells.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected ATF1 in HeLa cells.

Affinity Binding Assay: A representative lot of this antibody bound with ATF1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated ATF1 in lysate from HeLa nuclear extract.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1N21 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects ATF1. It targets an epitope within 15 amino acids from the N-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.