Anti-PIK3C3 Antibody, clone 1B9 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Phosphatidylinositol 3-kinase catalytic subunit type 3 (UniProt: Q8NEB9; also known as EC:2.7.1.137, PI3-kinase type 3;PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34 ) is encoded by the PIK3C3 (also known as  VPS34) gene (Gene ID: 5289) in human. It is a member of the PI 3-kinase family that mediates the active diversion of proteins from the secretory pathway to vacuoles. PIK3C3 is involved in both receptor-mediated signal transduction and intracellular trafficking. It enables 1-phosphatidylinositol-3-kinase activity and is Involved in several processes, including early endosome to late endosome transport, macroautophagy, and phosphatidylinositol-3-phosphate biosynthetic process. PIK3C3 acts upstream of or within autophagy and protein lipidation. It mediates catalysis on membranes and suppress futile adenosine triphosphatase cycles. Subcellularly, it is located in autolysosome, late endosome, and midbody. The protein appears to alternate between a closed cytosolic form and an open form on the membrane. The protein is ubiquitously expressed, with a highest expression in skeletal muscle. Dysregulation of PIK3C3 has been implicated in several diseases including glioma and also, acts as a critical driver in Cowden Syndrome (CS) and Cowden-like syndrome (CSL), highlighting its significance in oncogenic processes. Recent studies have elucidated its role in modulating cellular responses to stress and nutrient availability, further establishing PIK3C3 as a pivotal player in maintaining cellular homeostasis and implicating it in various pathologies associated with aberrant autophagic activity. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human PIK3C3.

Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate. 

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PIK3C3 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected PIK3C3 in lysates from C6 cells and human brain tissue and, a 1:10,000 dilution from a representative lot detected GST-tagged, full-length recombinant human PIK3C3.
Affinity Binding Assay: A representative lot of this antibody bound PIK3C3 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.                                   
Enzyme Immunoassay (ELISA): A representative lot of this antibody detected PIK3C3 protein in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1B9  is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects PIK3C3. It targets an epitope within 24 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.