Anti-POU3F2 Antibody, clone 1O12 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them. 
POU domain, class 3, transcription factor 2 (UniProt: P20265; also known as Brain-specific homeobox/POU domain protein 2 (Brain-2; Brn-2), Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7 (Oct-7), Octamer-binding transcription factor 7 (OTF-7)) is encoded by the POU3F2 (also known as BRN2, OCT7, OTF7) gene (Gene ID: 5454) in human. The POU domain, class 3, transcription factor 2 (POU3F2), also known as Brn-2, is a member of the POU-domain family of transcription factors, characterized by a bipartite DNA-binding domain comprising the POU-specific (POUS) and POU-homeodomain (POUH) regions. POU3F2 plays a critical role in neurogenesis and neuronal differentiation, particularly in the development of the hypothalamus and cortex. It binds to a specific octamer DNA sequence (5′-ATGCAT-3′) and regulates the expression of genes involved in neural development. POU3F2 is predominantly localized in the nucleus and is highly expressed in neural tissues, particularly in progenitor cells and differentiated neurons. It interacts with other neural transcription factors, such as SOX11, SOX4, and SOX2, to synergistically regulate target gene expression. POU3F2 is involved in several key pathways, including the Notch signaling cascade and the regulation of corticotropin-releasing hormone (CRH) genes, which are essential for stress response and hypothalamic function. Mutations or dysregulation of POU3F2 have been implicated in neurodevelopmental disorders and glioblastoma, where it contributes to tumor progression by promoting neural stem-like properties. Its role in epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) maintenance has also been noted, linking it to metastasis and therapy resistance in cancers. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human POU3F2.

Quality Control Testing
Evaluated by Western Blotting in SK-N-MC cell lysate. 

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected POU3F2 in SK-N-MC cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected POU3F2 in human brain tissue, mouse brain tissue, and rat brain tissue lysates.
Immunocytochemistry Analysis: A 1:1,000 Dilution from a representative lot detected POU3F2 in U-251 MG cells.
Affinity Binding Assay: A representative lot of this antibody bound with POU3F2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected POU3F2 in mouse brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1O12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects POU3F2. It targets an epitope within 17 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.