Anti-TOP2B Antibody, clone 5E7-H1 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
DNA topoisomerase 2-beta (UniProt: Q02880; also known as DNA topoisomerase II, beta isozyme, EC:5.6.2.2) is encoded by the TOP2B gene (Gene ID: 7155) in human. The TOP2B gene is located on human chromosome location 3p24.2. Topoisomerase IIβ (TOP2B) is an isoform of type IIA topoisomerases. It is a member of the topoisomerase II family and is a Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. It also plays a role in B-cell differentiation. Structurally, TOP2B consists of an N-terminal domain that facilitates DNA binding and a C-terminal domain that harbors ATPase activity, enabling the enzyme to introduce double-strand breaks in DNA to alleviate torsional strain. TOP2B is predominantly expressed in various tissues, including the brain, heart, and skeletal muscle, with notable localization in the nucleus, where it performs its role in DNA manipulation.  Two isoforms of TOP2B have been described that are produced by alternative splicing. TOP2B has been implicated in neurodegenerative disorders, as its dysfunction can disrupt neuronal homeostasis. Its involvement in disease is particularly significant in cancer, where aberrant expression or mutations in TOP2B can lead to genomic instability and contribute to tumorigenesis. Recent studies have highlighted the potential of TOP2B as a therapeutic target, particularly in the context of chemotherapy, where its inhibitors are used to enhance the efficacy of anticancer agents. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human TOP2B.

Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate. 

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOP2B in SH-SY5Y cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TOP2B in human kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound TOP2B peptide with at least one billion-fold (1,000,000,000X) higher affinity than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TOP2B in NIH/3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 5E7-H1 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects TOP2B. It targets an epitope within 20 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.