Anti-RAN Antibody, clone 1D6 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

GTP-binding nuclear protein Ran (UniProt: P62826; also known as Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein) is encoded by the RAN (also known as ARA24, OK/SW-cl.81) gene (Gene ID: 5901) in human. Ran is a small GTP binding protein of the RAS superfamily that is predominantly expressed in the nucleus during interphase and is dispersed throughout the cytoplasm during mitosis. The GTP-bound Ran (RanGTP) is considered active, while the GDP-bound Ran (RanGDP) is inactive. These two alternate states are regulated by two classes of partner proteins that either promote the RanGTP or the RanGDP form. RanGEF (guanine-nucleotide exchange factor, RCC1) enhances the formation of RanGTP and RanGAP (GTPase-activating protein) and RanBPs (Ran-binding proteins: RanBP1 and RanBP2), which increase the GTPase activity of Ran promote the formation of RanGDP. The GTPase activity of Ran is involved in nucleocytoplasmic transport, participating both to the import and the export of proteins and RNAs from the nucleus. Ran is shown to control cargo loading and release by transport receptors, such as importin , in the proper compartment, which ensures the directionality of the transport. Its interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. In its GTP-bound form RAN also triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fu, J., et al. (2010). Nature Edu. 3(9); 32; Melchior, F., et al. (1993). J. Cell Biol. 123(6 pt2); 1649-1659; Ren, M., et al. (1993). J. Cell Biol. 120(2); 313-323).

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human RAN.

Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RAN in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected RAN in lysates from NIH/3T3 cells, A-431 cells, and rat kidney tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RAN in human colon tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAN in PC-3 cells.

Affinity Binding Assay: A representative lot of this antibody bound RAN peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects RAN. It targets an epitope within 22 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.