505013
Discovery® RP-Amide C16 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
15 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
amide, alkyl phase
Pore size:
180 Å
Matrix:
silica gel, high purity, spherical base material
Product Name
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
agency
suitable for USP L60
Quality Level
feature
endcapped
manufacturer/tradename
Discovery®
extent of labeling
11% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
15 cm × 4.6 mm
surface area
200 m2/g
surface coverage
2.6 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical base material
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.
Application
Discovery® RP-AmideC16 HPLC column may be used for the separation of:
- Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
- Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
- Hydrophilic bases by LC with principal component analysis (PCA).
- 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.
Discovery RP-AmideC16 HPLC Column may be used for the separation of:
- Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
- Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
- Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
- Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.
Features and Benefits
- Excellent retention and resolution of polar compounds
- Unique selectivity compared to C18
- Excellent peak shape and efficiency
- Less hydrophobic than C18 columns
- Compatible with 100% aqueous mobile phases
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns
Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
HPLC separations of zidovudine and selected pharmaceuticals using a hexadecylsilane amide column.
Caufield WV and Stewart JT
Chromatographia, 54(9-10), 561-568 (2001)
Determination of vitamin B6 compounds in foods using liquid chromatography with post-column derivatization fluorescence detection.
Vinas P, et al.
Chromatographia, 59(5-6), 381-386 (2004)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 505013 | 04061838086792 |