54808
Mono S® 5/50 GL
10 μm particle size, L × I.D. 5 cm × 5 mm
Synonym(s):
Amersham Biosciences Ion Exchange Columns
L × I.D.
5 cm × 5 mm
matrix
MonoBeads
matrix active group
sulfonic acid
particle size
10 μm
compatibility
mode of use ion exchange
General description
Mono columns are highly efficient, pH-stable columns designed for high performance ion exchange separations of proteins, peptides, and polynucleotides, in applications including peptide mapping and monoclonal antibody purification. The unique properties of these columns are based on MonoBeads support – a beaded hydrophilic material with the narrowest particle size distribution of any chromatographic support. This monodispersity permits high flow rates at relatively low backpressures.
Application
Mono columns are used in applications including peptide mapping and monoclonal antibody purification. Mono S columns have been used in cancer research studies.
Legal Information
Mono S is a registered trademark of Cytiva
Regulatory Information
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Xiu-Juan Ye et al.
Journal of agricultural and food chemistry, 59(18), 10232-10238 (2011-08-13)
A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange
Evandro Fei Fang et al.
Archives of toxicology, 86(2), 293-304 (2011-10-05)
A 20-kDa Kunitz-type trypsin-chymotrypsin inhibitor, Bauhinia purpurea trypsin inhibitor (BPLTI), has been isolated from the seeds of B. purpurea L. by using liquid chromatography procedures that involved ion exchange chromatography on Sp-Sepharose and Mono S and gel filtration on Superdex
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